Composite

Part:BBa_K1740004

Designed by: Keisuke Sugahara   Group: iGEM15_KAIT_Japan   (2015-09-06)

pLac+RBS+145N+MCS+145N+stop codon

This part contains the coding sequence for Dronpa 145N and Multi Cloning Sites(MCS). This is designed so that Dronpa 145N is tagged to both terminus of the target protein. MCS contains the cleavage site of next restriction enzymes: Xho I, Hind III, Nde I, Kpn I, Sal I, Sac I and BamH I. We can obtain a fusion protein to react with A target DNA fragment which reacted their restriction enzymes and this part that treated restriction enzyme same as the fragment. The target protein activity in the fusion protein can control by light. But, this part could not make the deadline.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 959
    Illegal XhoI site found at 899
    Illegal XhoI site found at 911
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None